Signalling

Part:BBa_I13207

Designed by: Barry Canton   Group: Antiquity   (2004-08-01)


HSL/aiiA test construct

LuxR receiver circuit (driven by ptet) with pBad controlling the expression of aiiA.

Useful for measuring the ability of aiiA (LVA+) to turn off the response of a tet driven receiver cell to exogenous HSL levels

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3160
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3099
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2934
    Illegal AgeI site found at 3290
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004
    Illegal SapI site found at 2916


[edit]
Categories
//function/cellsignalling
Parameters
None